Messi returned to training this week after recovering from a right calf injury picked up in preseason, but the Argentina star was not included in the list of players available for the team’s home opener at Camp Nou Stadium.
He missed the team’s 1-0 loss to Athletic Bilbao in the league opener last weekend.
Coach Ernesto Valverde is also without forwards Luiz Suarez and Ousmane Dembele because of injuries.
Here’s how to watch all tonight’s action free of charge…
TV channel and live stream information
There was no buyer when rights for LaLiga were auctioned in July, but ITV confirmed one day before the season started that they would show one live game in each of the first three matchdays.
The match will be screened live for free on ITV4, and can be streamed online via the ITV Hub.
You can follow all the action and analysis on Standard Sport‘s LIVE blog with Ben Hayward.